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onlygoodantibodies.co.uk/tools/validation-recorder
A structured transparency record of antibody use for manuscript or grant submission. Enter each primary antibody once, then add application rows for each technique used.
The Validation Recorder is a structured data entry tool with wide tables and keyboard navigation. It works best on a laptop or desktop screen.
For mobile, download the offline version:
This record documents antibody use for transparency. Use Table 1 to enter each primary antibody's identity once. Use Table 2 to add one row for each antibody–application combination. Do not include secondary antibodies, isotype controls, or loading controls (e.g. β-actin).
RRID — Research Resource Identifier. Format: AB_ followed by digits. Entering a valid RRID will autofill vendor, catalogue number, host/clonality, clone, and target from the Antibody Registry.
Target — the protein, antigen, or marker. Start typing to see suggestions from the OGA gene list.
Vendor — the antibody supplier. Select from common vendors or type your own.
Catalogue # — the supplier's product number.
Product URL — link to the product page on the supplier's website.
Clone — clone designation for monoclonal antibodies.
Lot — batch or lot number.
Host / clonality — species and monoclonal/polyclonal status.
Independent characterisation — published or database evidence of this antibody's performance. Provide a URL or DOI.
Antibody — select which antibody from Table 1 this application row refers to.
Application — which technique was used. Each application gets its own row.
Sample — the biological material used.
Figures — which manuscript figures include data from this antibody.
Concentration — working concentration used. Often differs between applications.
These fields ask about controls for antibody specificity — not experimental controls for your treatment or condition. It is fine to leave these blank if no specificity controls were used; honest reporting is the goal.
Positive control — was a sample included where the antibody's target is known to be present? e.g. a cell line with confirmed expression, recombinant protein, or tissue with well-documented expression.
Negative control — was a sample included where the target is known to be absent? e.g. knockout/knockdown sample, secondary-only control, peptide competition.
Antibody-independent readouts — was the same target protein measured by a non-antibody method (e.g. mass spectrometry) with concordant results? Note: RT-qPCR measures mRNA, not protein, so only counts if correlation with antibody signal is explicitly demonstrated.
Control figs — which manuscript figures show your control data? If not in any figure, you can upload images at export.